SPOT-it™ TREC & KREC Screening Kit

SPOT-it™ TREC & SMN1 Screening Kit is an in vitro diagnostic kit intended for simultaneous screening for Severe Combined Immunodeficiency (SCID) and Spinal Muscular Atrophy (SMA) in newborns.

SCID screening is performed by semi-quantification of T-cell receptor excision circle (TREC) and SMA screening by a qualitative detection of exon 7 of the SMN1 gene. DNA is extracted from fresh, dried blood spots (DBS) sampled on filter paper. The principle of the assay is real-time quantitative polymerase chain reaction (qPCR).

The test is intended for use by laboratory professionals to aid in identifying newborns who potentially suffer from SCID caused by a lack of mature T-lymphocytes or SMA caused by homozygous exon 7 deletion in the SMN1 gene. Diagnosis shall be made by healthcare professionals through confirmatory testing, according to local requirements or recommendations.

Quick Assay Information

· Complete solution for SCID and SMA screening - including DNA extraction
· From Dried Blood Spot (DBS) to screening result within 3 hours
· Ready-to-use plates pre-filled with reaction mix and samples to generate a standard curve
· Just two pipetting steps for quick and easy handling
· Uses conventional 3.2 mm ⌀ DBS as input
· Designed for the needs of screening laboratories
· CE marked - For In Vitro Diagnostic (IVD) use in EU

How it works

Sample distribution

Dried Blood Spot (DBS) samples
are punched into SPOT-it™ filter plate.

Rinse DBS

Samples are rinsed and rehydrated to remove hemoglobin and other impurities.

DNA Elution

DNA is eluted by heating to 95 °C for
30 minutes.


TREC and ACTB copies are quantified and SMN1 detected using qPCR.

Product Details

Product name:

SPOT-it™ TREC & SMN1 Screening Kit


Newborn screening for SCID & SMA

Product Code





1 x 96-well plate

4 x 96-well plate

12 x 96-well plates

Samples per kit