SPOT-it™ TREC & KREC Screening Kit

SPOT-it™ TREC & KREC Screening Kit is an in vitro diagnostic (IVD) kit intended for newborn screening for severe combined immunodeficiency (SCID) and agammaglobulinemia (XLA). The kit quantifies T-cell receptor excision circle (TREC) DNA and kappa-deleting recombination excision circles (KREC) DNA extracted from fresh, prospective dried blood spot (DBS) sampled on filter paper. The analysis is performed by semi-quantitative real-time polymerase chain reaction (qPCR).

The test is intended for use by laboratory professionals to aid in identifying newborns who potentially suffer from SCID caused by lack of mature T- or T and B-cells or XLA caused by lack of mature B-cells.

TRECs and KRECs are produced during the maturation of T-cells and B-cells respectively — vital components of the immune system. Lack of TRECs and KRECs indicate low or no production of mature T- and B-cells. A newborn identified with low TRECs or with low TRECs and KRECs can potentially suffer from SCID, while a newborn identified with low KRECs can potentially suffer from XLA. Confirmatory testing should be performed before diagnosis.

Quick Assay Information

· Complete solution for SCID screening - including DNA extraction
· From Dried Blood Spot (DBS) to screening result within 3 hours
· Ready-to-use plates pre-filled with reaction mix and samples to generate a standard curve
· Just two pipetting steps for quick and easy handling
· Uses conventional 3.2 mm ⌀ DBS as input
· Designed for the needs of screening laboratories
· CE marked - For In Vitro Diagnostic (IVD) use in EU

How it works

Sample distribution

Dried Blood Spot (DBS) samples
are punched into SPOT-it™ filter plate.

Rinse DBS

Samples are rinsed and rehydrated to remove hemoglobin and other impurities.

DNA Elution

DNA is eluted by heating to 95 °C for
30 minutes.


TREC, KREC and ACTB copies are quantified using qPCR

Product Details

Product name:

SPOT-it™ TREC & KREC Screening Kit


Newborn screening for SCID & XLA

Product Code




1 x 96-well plate

12 x 96-well plate

Samples per kit