Our CE IVD Products

SPOT-itTM
TREC
Screening Kit

SPOT-it™ TREC Screening kit quantifies TREC and ACTB in a sample using qPCR.

About

Newborn screening for Severe Combined Immunodeficiency (SCID).

The SPOT-it™ TREC Screening kit detects two markers: T-cell receptor excision circles (TREC) and Beta-actin (ACTB).

Semi-quantification of TREC is used to identify potential cases of SCID, as TRECs are circular DNA fragments formed at the early stages of T-lymphocytes (T-cells) maturation — a critical process for immune system development. Thus, low or undetectable levels of TREC in newborn’s blood are highly indicative of SCID.

ACTB gene, coding for Beta-actin protein, functions as an internal control in the assay, ensuring sample quality and accuracy of results.

Diagnosis shall be made by healthcare professionals through confirmatory testing, according to local requirements or recommendations.

Quick Assay
Information

SPOT-it™ TREC
Screening Kits

Reliable and accessible in vitro diagnostic assays manufactured in Sweden

01

qPCR test method

Real-time quantitative polymerase chain reaction (qPCR) assay principle.

02

Quick procedure

From Dried Blood Spot (DBS) to screening result within 3 hours, with no manual samples transfer needed.

03

Easy handling

Liquid transfer through innovative plate-stacking method and ready-to-use, pre-filled reagent plates.

04

No need for separate clean room

The use of ready-to-use, pre-filled reagent plates removes the need for a separate clean room for MasterMix preparation.

05

Trusted kits

Used in national screening programs in multiple European countries since 2017.

06

CE-marked

Certified for in vitro diagnostic use in Europe.

Discover how the assay works

The test is intended for use by laboratory professionals to aid in identifying newborns, who potentially suffer from SCID.
01

Sample distribution

Distribute a 3.2 mm diameter punch from each Dried Blood Spot (DBS) sample into the SPOT-it™ Filter Plate.

02

Rinse Dried Blood Spots

Rinse and rehydrate the samples to remove hemoglobin and other impurities.

03

Elution of the DNA

Transfer rinsed punches into the pre-filled Elution Plate using centrifugation and plate-stacking technology. DNA is then eluted by the heating process.

04

qPCR Amplification

Transfer the eluted DNA to the pre-filled qPCR Plate using centrifugation and plate-stacking technology; and launch the qPCR run.

05

Result Interpretation

Understand results rapidly by visualizing the data, and verify control parameters.

With SPOT-it™ kits, SCID screening is made accessible to laboratories of all sizes, as we eliminate the need for costly automation equipment, thanks to our innovative plate-stacking technology.
Erna Domsgen

CEO at ImmunoIVD

Product details

Product name:

SPOT-itTM TREC Screening Kit

Description:

Newborn screening for SCID

Product Code Format Samples per kit
4-2015-T 4 x 96-well plate 328
12-2015-T 12 x 96-well plate 984

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