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SPOT-itTM
TREC & KREC
Screening Kit

SPOT-it™ TREC & KREC Screening kit is an in vitro diagnostic (IVD) kit intended for newborn screening for severe combined immunodeficiency (SCID) and agammaglobulinemia (XLA).

The kit identifies newborns potentially affected by SCID, characterized by a lack of mature T-cells or both T- and B-cells, and XLA, marked by an absence of mature B-cells.

Multiplex Screening

Newborn screening for SCID and XLA from a single Dried Blood Spot (DBS) punch.

The SPOT-it™ TREC & KREC Screening kit detects three essential markers — TREC, KREC, and Beta-actin (ACTB) — to screen for Severe Combined Immunodeficiency (SCID) and X-linked Agammaglobulinemia (XLA) using a single DBS punch.

Newborns with certain immunodeficiencies, including SCID, often show a significant reduction or absence of TREC copies, making TREC a key indicator for SCID.

KRECs, produced during B-cell maturation, provide insights into B-cell levels. A lack of KRECs alongside low TREC levels suggests SCID affecting both T- and B-cells, while a low KREC level with normal TREC may indicate XLA.

The ACTB gene, encoding Beta-actin protein, serves as an internal control to verify sample quality and assay performance.

Diagnosis shall be made by healthcare professionals through confirmatory testing, according to local requirements or recommendations.

Quick Assay
Information

SPOT-itTM
TREC & KREC
Screening Kits

Reliable and accessible in vitro diagnostic assays manufactured in Sweden

01

Multiplex qPCR test method

Multiplex SCID and XLA screening in one assay with real-time PCR technology.

02

Quick procedure

From Dried Blood Spot (DBS) to screening result within 3 hours, with no manual samples transfer needed.

03

Easy handling

Innovative plate-stacking method enables efficient liquid transfer.

04

No need for separate clean room

No need for separate clean room for MasterMix preparation.

05

Trusted kits

Used in national screening programs in multiple European countries since 2018.

06

CE-marked

Certified for in vitro diagnostic use in Europe.

Discover how the assay works

The test is intended for use by laboratory professionals to aid in identifying newborns, who potentially suffer from SCID and XLA.
01

Sample distribution

Distribute a 3.2 mm diameter punch from each Dried Blood Spot (DBS) sample into the SPOT-it™ Filter Plate.

02

Rinse Dried Blood Spots

Samples are rinsed and rehydrated to remove hemoglobin and other impurities.

03

Elution of the DNA

Transfer rinsed punches into the pre-filled Elution Plate using centrifugation and plate-stacking technology. DNA is then eluted by the heating process.

04

qPCR Amplification

Transfer the eluted DNA to the pre-filled qPCR Plate using centrifugation and plate-stacking technology; and launch the qPCR run.

05

Result Interpretation

Understand results rapidly by visualizing the data, and verify control parameters.

SCID and XLA screenings are performed by semi-quantification of T-TREC and KREC. DNA is extracted from 3.2 mm fresh, dried blood spot (DBS) sampled on filter paper.
Erna Domsgen, PhD

CEO at ImmunoIVD

Product details

Product name:

SPOT-it™ TREC & KREC Screening Kit

Description:

Simultaneous screening for SCID and XLA

Product Code Format Samples per kit
4-2015-TK 4 x 96-well plate 324
12-2015-TK 12 x 96-well plate 972

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